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Mechanistic evaluation of the pros and cons of digital RT-LAMP for HIV-1 viral load quantification on a microfluidic device and improved efficiency via a two-step digital protocol

Here we used a SlipChip microfluidic device to evaluate the performance of digital reverse transcription-loop mediated isothermal amplification (dRT-LAMP) for quantification of HIV viral RNA. Tests are needed for monitoring HIV viral load to control the emergence of drug resistance and to diagnose a...

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Detalhes bibliográficos
Main Authors: Sun, Bing, Shen, Feng, McCalla, Stephanie E., Kreutz, Jason E., Karymov, Mikhail A., Ismagilov, Rustem F.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3578705/
https://ncbi.nlm.nih.gov/pubmed/23324061
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac3037206
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