The Single T65S Mutation Generates Brighter Cyan Fluorescent Proteins with Increased Photostability and pH Insensitivity

Cyan fluorescent proteins (CFP) derived from Aequorea victoria GFP, carrying a tryptophan-based chromophore, are widely used as FRET donors in live cell fluorescence imaging experiments. Recently, several CFP variants with near-ultimate photophysical performances were obtained through a mix of site-...

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Main Authors: Fredj, Asma, Pasquier, Hélène, Demachy, Isabelle, Jonasson, Gabriella, Levy, Bernard, Derrien, Valérie, Bousmah, Yasmina, Manoussaris, Gallia, Wien, Frank, Ridard, Jacqueline, Erard, Marie, Merola, Fabienne
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2012
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3487735/
https://ncbi.nlm.nih.gov/pubmed/23133673
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0049149
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