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Optimization of transient gene expression in mammalian cells and potential for scale-up using flow electroporation

The goals of this study were to identify mammalian cell lines which could be efficiently transiently-transfected and scaled-up for protein production. The transfection efficiencies of eight cell lines (NSO, NSO-TAg, CV-1, COS-7, CHO, CHO-TAg, HEK 293, and 293-EBNA) were measured using electroporatio...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Parham, Janet H., Iannone, Marie A., Overton, Laurie K., Hutchins, Jeff T.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Kluwer Academic Publishers 1998
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC3449836/
https://ncbi.nlm.nih.gov/pubmed/19003416
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1008046101653
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