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Optimization of transient gene expression in mammalian cells and potential for scale-up using flow electroporation
The goals of this study were to identify mammalian cell lines which could be efficiently transiently-transfected and scaled-up for protein production. The transfection efficiencies of eight cell lines (NSO, NSO-TAg, CV-1, COS-7, CHO, CHO-TAg, HEK 293, and 293-EBNA) were measured using electroporatio...
Gorde:
Egile Nagusiak: | , , , |
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Formatua: | Artigo |
Hizkuntza: | Inglês |
Argitaratua: |
Kluwer Academic Publishers
1998
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Gaiak: | |
Sarrera elektronikoa: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3449836/ https://ncbi.nlm.nih.gov/pubmed/19003416 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1008046101653 |
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