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Optimization of transient gene expression in mammalian cells and potential for scale-up using flow electroporation
The goals of this study were to identify mammalian cell lines which could be efficiently transiently-transfected and scaled-up for protein production. The transfection efficiencies of eight cell lines (NSO, NSO-TAg, CV-1, COS-7, CHO, CHO-TAg, HEK 293, and 293-EBNA) were measured using electroporatio...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Kluwer Academic Publishers
1998
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3449836/ https://ncbi.nlm.nih.gov/pubmed/19003416 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1008046101653 |
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