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Non-GMP plasmid production for transient transfection in bioreactors
We describe a generic plasmid purification process for producing DNA for larger-scale transient transfection. Data on plasmid quality with regard to residual protein, endotoxin content and presence of different plasmid forms is given. The effects of contaminants and plasmid forms on expression level...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Kluwer Academic Publishers
2001
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3449703/ https://ncbi.nlm.nih.gov/pubmed/22358854 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1013148203049 |
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