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Influence of monovalent cations on the activity of T4 DNA ligase in the presence of polyethylene glycol.
Monovalent cations such as Na+ and K+ inhibit the activity of T4 DNA ligase. However, the extent of inhibition varies with the terminal sequence of the duplex DNA used as substrate; in many cases, ligation of DNA is completely inhibited at 200 mM. The activity of the ligase is stimulated by raising...
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| Autors principals: | , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
1985
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| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC341233/ https://ncbi.nlm.nih.gov/pubmed/2987879 |
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