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An Image Analysis Method for the Precise Selection and Quantitation of Fluorescently Labeled Cellular Constituents: Application to the Measurement of Human Muscle Cells in Culture

The accurate measurement of the morphological characteristics of cells with nonuniform conformations presents difficulties. We report here a straightforward method using immunofluorescent staining and the commercially available imaging program Adobe Photoshop, which allows objective and precise info...

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Detalhes bibliográficos
Main Authors: Agley, Chibeza C., Velloso, Cristiana P., Lazarus, Norman R., Harridge, Stephen D. R.
Formato: Artigo
Idioma:Inglês
Publicado em: SAGE Publications 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3393072/
https://ncbi.nlm.nih.gov/pubmed/22511600
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1369/0022155412442897
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