in vivo protein trapping produces a functional expression codex of the vertebrate proteome

We describe a conditional in vivo protein trap mutagenesis system that reveals spatio-temporal protein expression dynamics and assesses gene function in the vertebrate Danio rerio. Integration of pGBT-RP2 (RP2), a gene-breaking transposon containing a protein trap, efficiently disrupts gene expressi...

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Bibliographic Details
Main Authors: Clark, Karl J., Balciunas, Darius, Pogoda, Hans-Martin, Ding, Yonghe, Westcot, Stephanie E., Bedell, Victoria M., Greenwood, Tammy M., Urban, Mark D., Skuster, Kimberly J., Petzold, Andrew M., Ni, Jun, Nielsen, Aubrey, Patowary, Ashok, Scaria, Vinod, Sivasubbu, Sridhar, Xu, Xiaolei, Hammerschmidt, Matthias, Ekker, Stephen C.
Format: Artigo
Language:Inglês
Published: 2011
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3306164/
https://ncbi.nlm.nih.gov/pubmed/21552255
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1606
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