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Amplified insert assembly: an optimized approach to standard assembly of BioBrick(TM) genetic circuits
A modified BioBrick™ assembly method was developed with higher fidelity than current protocols. The method utilizes a PCR reaction with a standard primer set to amplify the inserted part. Background colonies are reduced by a combination of dephosphorylation and digestion with DpnI restriction endonu...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
BioMed Central
2011
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3287150/ https://ncbi.nlm.nih.gov/pubmed/22176971 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1754-1611-5-17 |
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