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Amplified insert assembly: an optimized approach to standard assembly of BioBrick(TM) genetic circuits

A modified BioBrick™ assembly method was developed with higher fidelity than current protocols. The method utilizes a PCR reaction with a standard primer set to amplify the inserted part. Background colonies are reduced by a combination of dephosphorylation and digestion with DpnI restriction endonu...

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Hlavní autoři: Speer, Michael A, Richard, Tom L
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2011
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3287150/
https://ncbi.nlm.nih.gov/pubmed/22176971
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1754-1611-5-17
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