Development of a 16S rRNA Gene Primer and PCR-Restriction Fragment Length Polymorphism Method for Rapid Detection of Members of the Genus Megasphaera and Species-Level Identification

The genus Megasphaera is relevant to the environment, human health and food, and renewable energy for the future. In this study, a primer set was designed for PCR-restriction fragment length polymorphism (RFLP) analyses to detect and identify the members of Megasphaera. Direct detection and identifi...

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Detalhes bibliográficos
Main Authors: Ohnishi, Akihiro, Abe, Shinko, Nashirozawa, Shiho, Shimada, Sayaka, Fujimoto, Naoshi, Suzuki, Masaharu
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2011
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3147432/
https://ncbi.nlm.nih.gov/pubmed/21705538
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00359-11
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