Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19

Cwp19 is a putatively surface-located protein from Clostridium difficile. A recombinant N-terminal protein (residues 27–401) lacking the signal peptide and the C-terminal cell-wall-binding repeats (PFam04122) was crystallized using the sitting-drop vapour-diffusion method and diffracted to 2 Å resol...

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Bibliographic Details
Main Authors: Kirby, Jonathan M., Thiyagarajan, Nethaji, Roberts, April K., Shone, Clifford C., Acharya, K. Ravi
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2011
Subjects:
Crystallization Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3144791/
https://ncbi.nlm.nih.gov/pubmed/21795789
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111016770
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