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A method for the quantitative analysis of human heat shock gene expression using a multiplex RT-PCR assay

A quantitative multiplex RT-PCR assay is described to measure the levels of messenger RNAs for eight human genes encoding the heat shock proteins (HSP) and molecular chaperones hsp90α, hsp90β, hsp70, hsc70, mtHsp75, Grp78 (BiP), hsp60 and hsp27. The basis of this assay is reverse transcription of to...

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Main Authors: Wang, San Ming, Khandekar, Janardan D., Kaul, Karen L., Winchester, David J., Morimoto, Richard I.
Format: Artigo
Jezik:Inglês
Izdano: Cell Stress Society International 1999
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC312929/
https://ncbi.nlm.nih.gov/pubmed/10547064
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