Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc

BACKGROUND: The accuracy of quantitative real-time RT-PCR (qRT-PCR) is often influenced by experimental artifacts, resulting in erroneous expression profiles of target genes. The practice of employing normalization using a reference gene significantly improves reliability and its applicability to mo...

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Main Authors: Seol, Dongrim, Choe, Hyeonghun, Zheng, Hongjun, Jang, Keewoong, Ramakrishnan, Prem S, Lim, Tae-Hong, Martin, James A
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2011
Assuntos:
Short Report
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3118343/
https://ncbi.nlm.nih.gov/pubmed/21615931
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-4-162
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