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Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc
BACKGROUND: The accuracy of quantitative real-time RT-PCR (qRT-PCR) is often influenced by experimental artifacts, resulting in erroneous expression profiles of target genes. The practice of employing normalization using a reference gene significantly improves reliability and its applicability to mo...
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Main Authors: | , , , , , , |
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Format: | Artigo |
Language: | Inglês |
Published: |
BioMed Central
2011
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Subjects: | |
Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3118343/ https://ncbi.nlm.nih.gov/pubmed/21615931 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-4-162 |
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