A new implementation of high-throughput five-dimensional clone pooling strategy for BAC library screening

BACKGROUND: A five-dimensional (5-D) clone pooling strategy for screening of bacterial artificial chromosome (BAC) clones with molecular markers utilizing highly-parallel Illumina GoldenGate assays and PCR facilitates high-throughput BAC clone and BAC contig anchoring on a genetic map. However, this...

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Autores principales: You, Frank M, Luo, Ming-Cheng, Xu, Kenong, Deal, Karin R, Anderson, Olin D, Dvorak, Jan
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2010
Materias:
Methodology Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3016418/
https://ncbi.nlm.nih.gov/pubmed/21129228
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-11-692
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