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Allelic Exchange in Actinomyces oris with mCherry Fluorescence Counterselection
Described here is a method for facile generation of markerless gene deletion mutants of Actinomyces oris. Homologous integration of a nonreplicative vector carrying a gene exchange cassette into the bacterial chromosome was selected for by using mCherry fluorescence and resistance to kanamycin. Comp...
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| Autori principali: | , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
American Society for Microbiology (ASM)
2010
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2935053/ https://ncbi.nlm.nih.gov/pubmed/20601506 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00811-10 |
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