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The Effects of Alignment Quality, Distance Calculation Method, Sequence Filtering, and Region on the Analysis of 16S rRNA Gene-Based Studies

Pyrosequencing of PCR-amplified fragments that target variable regions within the 16S rRNA gene has quickly become a powerful method for analyzing the membership and structure of microbial communities. This approach has revealed and introduced questions that were not fully appreciated by those carry...

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Autore principale: Schloss, Patrick D.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2010
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2900292/
https://ncbi.nlm.nih.gov/pubmed/20628621
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pcbi.1000844
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