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The Effects of Alignment Quality, Distance Calculation Method, Sequence Filtering, and Region on the Analysis of 16S rRNA Gene-Based Studies
Pyrosequencing of PCR-amplified fragments that target variable regions within the 16S rRNA gene has quickly become a powerful method for analyzing the membership and structure of microbial communities. This approach has revealed and introduced questions that were not fully appreciated by those carry...
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| Formato: | Artigo |
| Lenguaje: | Inglês |
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Public Library of Science
2010
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| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2900292/ https://ncbi.nlm.nih.gov/pubmed/20628621 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pcbi.1000844 |
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