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Crystallization and preliminary X-ray crystallographic studies of an exo-β-d-glucosaminidase from Trichoderma reesei

Chitosan is degraded to glucosamine (GlcN) by chitosanase and exo-β-d-glucosaminidase (GlcNase). GlcNase from Trichoderma reesei (Gls93) is a 93 kDa extracellular protein composed of 892 amino acids. The enzyme liberates GlcN from the nonreducing end of the chitosan chain in an exo-type manner and b...

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Bibliographische Detailangaben
Hauptverfasser: Sakamoto, Yasumitsu, Ike, Masakazu, Tanaka, Nobutada, Suzuki, Yoshiyuki, Ogasawara, Wataru, Okada, Hirofumi, Nonaka, Takamasa, Morikawa, Yasushi, Nakamura, Kazuo T.
Format: Artigo
Sprache:Inglês
Veröffentlicht: International Union of Crystallography 2010
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2833044/
https://ncbi.nlm.nih.gov/pubmed/20208168
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110000606
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