Coordination sphere of the third metal site is essential to the activity and metal selectivity of alkaline phosphatases

Alkaline phosphatases (APs) are commercially applied enzymes that catalyze the hydrolysis of phosphate monoesters by a reaction involving three active site metal ions. We have previously identified H135 as the key residue for controlling activity of the psychrophilic TAB5 AP (TAP). In this article,...

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Asıl Yazarlar: Koutsioulis, Dimitris, Lyskowski, Andrzej, Mäki, Seija, Guthrie, Ellen, Feller, Georges, Bouriotis, Vassilis, Heikinheimo, Pirkko
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Wiley Subscription Services, Inc., A Wiley Company 2010
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Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC2817841/
https://ncbi.nlm.nih.gov/pubmed/19916164
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.284
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