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Coordination sphere of the third metal site is essential to the activity and metal selectivity of alkaline phosphatases
Alkaline phosphatases (APs) are commercially applied enzymes that catalyze the hydrolysis of phosphate monoesters by a reaction involving three active site metal ions. We have previously identified H135 as the key residue for controlling activity of the psychrophilic TAB5 AP (TAP). In this article,...
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| Главные авторы: | , , , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Wiley Subscription Services, Inc., A Wiley Company
2010
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2817841/ https://ncbi.nlm.nih.gov/pubmed/19916164 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.284 |
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