DNA Extraction Procedures Meaningfully Influence qPCR-Based mtDNA Copy Number Determination

Quantitative real time PCR (qPCR) is commonly used to determine cell mitochondrial DNA (mtDNA) copy number. This technique involves obtaining the ratio of an unknown variable (number of copies of an mtDNA gene) to a known parameter (number of copies of a nuclear DNA gene) within a genomic DNA sample...

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Bibliografiske detaljer
Main Authors: Guo, Wen, Jiang, Lan, Bhasin, Shalender, Khan, Shaharyar M., Swerdlow, Russell H.
Format: Artigo
Sprog:Inglês
Udgivet: 2009
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2798162/
https://ncbi.nlm.nih.gov/pubmed/19324101
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mito.2009.03.003
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