BchY-Based Degenerate Primers Target All Types of Anoxygenic Photosynthetic Bacteria in a Single PCR

To detect anoxygenic bacteria containing either type 1 or type 2 photosynthetic reaction centers in a single PCR, we designed a degenerate primer set based on the bchY gene. The new primers were validated in silico using the GenBank nucleotide database as well as by PCR on pure strains and environme...

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Bibliographic Details
Main Authors: Yutin, Natalya, Suzuki, Marcelino T., Rosenberg, Mira, Rotem, Denisse, Madigan, Michael T., Süling, Jörg, Imhoff, Johannes F., Béjà, Oded
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2009
Subjects:
Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2786397/
https://ncbi.nlm.nih.gov/pubmed/19801482
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01014-09
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