BchY-Based Degenerate Primers Target All Types of Anoxygenic Photosynthetic Bacteria in a Single PCR

To detect anoxygenic bacteria containing either type 1 or type 2 photosynthetic reaction centers in a single PCR, we designed a degenerate primer set based on the bchY gene. The new primers were validated in silico using the GenBank nucleotide database as well as by PCR on pure strains and environme...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Yutin, Natalya, Suzuki, Marcelino T., Rosenberg, Mira, Rotem, Denisse, Madigan, Michael T., Süling, Jörg, Imhoff, Johannes F., Béjà, Oded
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: American Society for Microbiology (ASM) 2009
Pynciau:
Methods
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC2786397/
https://ncbi.nlm.nih.gov/pubmed/19801482
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01014-09
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