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Insights into branch nucleophile positioning and activation from an orthogonal pre-mRNA splicing system in yeast
The duplex formed between the branch site (BS) of a spliceosomal intron and its cognate sequence in U2 snRNA is important for spliceosome assembly and the first catalytic step of splicing. We describe the development of an orthogonal BS-U2 system in S. cerevisiae in which spliceosomes containing a g...
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| Auteurs principaux: | , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2009
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2730498/ https://ncbi.nlm.nih.gov/pubmed/19450531 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2009.03.012 |
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