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Live-Cell Imaging and Measurement of Intracellular pH in Filamentous Fungi Using a Genetically Encoded Ratiometric Probe

A novel, genetically encoded, ratiometric pH probe (RaVC) was constructed to image and measure intracellular pH in living hyphae of Aspergillus niger. RaVC is a chimeric protein based on the pH-sensitive probe pHluorin, which was partially codon optimized for expression in Aspergillus. Intracellular...

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Autors principals: Bagar, Tanja, Altenbach, Kirsten, Read, Nick D., Benčina, Mojca
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 2009
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2681602/
https://ncbi.nlm.nih.gov/pubmed/19286983
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/EC.00333-08
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