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Leucine/Valine Residues Direct Oxygenation of Linoleic Acid by (10R)- and (8R)-Dioxygenases: EXPRESSION AND SITE-DIRECTED MUTAGENESIS OF (10R)-DIOXYGENASE WITH EPOXYALCOHOL SYNTHASE ACTIVITY

Linoleate (10R)-dioxygenase (10R-DOX) of Aspergillus fumigatus was cloned and expressed in insect cells. Recombinant 10R-DOX oxidized 18:2n-6 to (10R)-hydroperoxy-8(E),12(Z)-octadecadienoic acid (10R-HPODE; ∼90%), (8R)-hydroperoxylinoleic acid (8R-HPODE; ∼10%), and small amounts of 12S(13R)-epoxy-(1...

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Detalhes bibliográficos
Main Authors:	Garscha, Ulrike, Oliw, Ernst H.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Biochemistry and Molecular Biology 2009
Assuntos:	Lipids and Lipoproteins: Metabolism, Regulation, and Signaling
Acesso em linha:	https://ncbi.nlm.nih.gov/pmc/articles/PMC2679477/ https://ncbi.nlm.nih.gov/pubmed/19289462 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M808665200
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Leucine/Valine Residues Direct Oxygenation of Linoleic Acid by (10R)- and (8R)-Dioxygenases: EXPRESSION AND SITE-DIRECTED MUTAGENESIS OF (10R)-DIOXYGENASE WITH EPOXYALCOHOL SYNTHASE ACTIVITY

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