Efficient mouse transgenesis using Gateway-compatible ROSA26 locus targeting vectors and F1 hybrid ES cells

The ability to rapidly and efficiently generate reliable Cre/loxP conditional transgenic mice would greatly complement global high-throughput gene targeting initiatives aimed at identifying gene function in the mouse. We report here the generation of Cre/loxP conditional ROSA26-targeted ES cells wit...

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Detalhes bibliográficos
Main Authors: Nyabi, Omar, Naessens, Michael, Haigh, Katharina, Gembarska, Agnieszka, Goossens, Steven, Maetens, Marion, De Clercq, Sarah, Drogat, Benjamin, Haenebalcke, Lieven, Bartunkova, Sonia, De Vos, Ilse, De Craene, Bram, Karimi, Mansour, Berx, Geert, Nagy, Andras, Hilson, Pierre, Marine, Jean-Christophe, Haigh, Jody J.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2009
Assuntos:
Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2673446/
https://ncbi.nlm.nih.gov/pubmed/19279185
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp112
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