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Development of a Rapid High-Throughput Method for High-Resolution Melting Analysis for Routine Detection and Genotyping of Noroviruses

We developed a simple, rapid, high-throughput detection and genotyping method for noroviruses using real-time reverse transcription-PCR (RT-PCR) and high-resolution melting (HRM) analysis to create a difference plot. The capsid gene was amplified by real-time RT-PCR in the presence of ResoLight HRM...

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Detalhes bibliográficos
Main Authors: Tajiri-Utagawa, Etsuko, Hara, Masayuki, Takahashi, Kuniaki, Watanabe, Mayumi, Wakita, Takaji
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2009
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2643702/
https://ncbi.nlm.nih.gov/pubmed/19073870
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01247-08
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