A New FRAP/FRAPa Method for Three-Dimensional Diffusion Measurements Based on Multiphoton Excitation Microscopy

We present a new convenient method for quantitative three-dimensionally resolved diffusion measurements based on the photobleaching (FRAP) or photoactivation (FRAPa) of a disk-shaped area by the scanning laser beam of a multiphoton microscope. Contrary to previously reported spot-photobleaching prot...

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Detalhes bibliográficos
Main Authors: Mazza, Davide, Braeckmans, Kevin, Cella, Francesca, Testa, Ilaria, Vercauteren, Dries, Demeester, Jo, De Smedt, Stefaan S., Diaspro, Alberto
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2008
Assuntos:
Spectroscopy, Imaging, Other Techniques
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2547427/
https://ncbi.nlm.nih.gov/pubmed/18621824
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.108.133637
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