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The funnel approach to the pre-crystallization production of membrane proteins

Challenges in the production of integral membrane proteins for structural studies include low expression levels, incorrect membrane insertion, aggregation and instability. In this report, we describe a “funnel approach” to overcome these difficulties, and demonstrate its efficacy in a case study of...

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Bibliografske podrobnosti
Main Authors: Lewinson, Oded, Lee, Allen T., Rees, Douglas C.
Format: Artigo
Jezik:Inglês
Izdano: 2008
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2362151/
https://ncbi.nlm.nih.gov/pubmed/18241890
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2007.12.059
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