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The funnel approach to the pre-crystallization production of membrane proteins
Challenges in the production of integral membrane proteins for structural studies include low expression levels, incorrect membrane insertion, aggregation and instability. In this report, we describe a “funnel approach” to overcome these difficulties, and demonstrate its efficacy in a case study of...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2008
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2362151/ https://ncbi.nlm.nih.gov/pubmed/18241890 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2007.12.059 |
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