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Simultaneous assignment and structure determination of a membrane protein from NMR orientational restraints
A solid-state NMR approach for simultaneous resonance assignment and three-dimensional structure determination of a membrane protein in lipid bilayers is described. The approach is based on the scattering, hence the descriptor “shotgun,” of (15)N-labeled amino acids throughout the protein sequence (...
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Main Authors: | , |
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Format: | Artigo |
Jezik: | Inglês |
Izdano: |
Cold Spring Harbor Laboratory Press
2003
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Teme: | |
Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2312444/ https://ncbi.nlm.nih.gov/pubmed/12592011 |
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