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Simultaneous assignment and structure determination of a membrane protein from NMR orientational restraints

A solid-state NMR approach for simultaneous resonance assignment and three-dimensional structure determination of a membrane protein in lipid bilayers is described. The approach is based on the scattering, hence the descriptor “shotgun,” of (15)N-labeled amino acids throughout the protein sequence (...

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Autors principals: Marassi, Francesca M., Opella, Stanley J.
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 2003
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2312444/
https://ncbi.nlm.nih.gov/pubmed/12592011
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