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Covalent attachment of enzyme as a membrane-label for viable eucaryotic cells
An enzyme, beta-D-galactosidase, was covalently coupled to mammalian cells by means of a bifunctional reagent. The coupling procedure did not cause appreciable loss of cell viability (less than 6%) as measured by plating efficiently and membrane integrity. After 24 h in culture, the cells exhibited...
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| Formato: | Artigo |
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| Idioma: | Inglês |
| Publicado em: |
The Rockefeller University Press
1979
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2111534/ https://ncbi.nlm.nih.gov/pubmed/115893 |
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