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ISOLATION AND PARTIAL CHARACTERIZATION OF MACRO- AND MICRONUCLEI FROM PARAMECIUM AURELIA
A method was developed for the isolation of macro- and micronuclei from Paramecium aurelia. This method utilized ionic and nonionic detergents to rupture the intact cells, calcium ions and spermidine were employed to protect the nuclei, and the nuclei were purified by centrifugation. Macronuclei con...
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| Main Author: | |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
The Rockefeller University Press
1972
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2108708/ https://ncbi.nlm.nih.gov/pubmed/4552140 |
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