Construction of a modified penicillin-binding protein 2a from methicillin-resistant Staphylococcus aureus and purification by immobilized metal affinity chromatography.

The mecA-27r gene, which encodes PBP2a-27r, was modified by site-specific mutagenesis, resulting in replacement of the N-terminal membrane anchor with a short chelating peptide (CP-PBP2a-27r). CP-PBP2a-27r retained the same binding affinity for beta-lactam antibiotics as the wild-type enzyme. Approx...

Popoln opis

Shranjeno v:
Bibliografske podrobnosti
Main Authors: Wu, C Y, Blaszczak, L C, Smith, M C, Skatrud, P L
Format: Artigo
Jezik:Inglês
Izdano: 1994
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC205227/
https://ncbi.nlm.nih.gov/pubmed/8113200
Oznake: Označite
Brez oznak, prvi označite!

Podobne knjige/članki