Molecular cloning of the pyrE gene from the extreme thermophile Thermus flavus.

Mutants of the extreme thermophile Thermus flavus in the pyrimidine biosynthetic pathway (Pyr-) were isolated by resistance to 5-fluoroorotic acid. The pyrE gene, which codes for the orotate phosphoribosyltransferase, was cloned by recombination with one of the isolated Pyr- T. flavus mutant strains...

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Main Authors: Vonstein, V, Johnson, S P, Yu, H, Casadaban, M J, Pagratis, N C, Weber, J M, Demirjian, D C
Format: Artigo
Jezik:Inglês
Izdano: 1995
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC177211/
https://ncbi.nlm.nih.gov/pubmed/7635839
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