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Cloning, nucleotide sequencing, and expression of an opine dehydrogenase gene from Arthrobacter sp. strain 1C.
The gene coding for opine dehydrogenase from Arthrobacter sp. strain 1C was cloned onto plasmid pBluescript KS(-), and the nucleotide sequence of the 1,077-bp open reading frame consisting of 359 codons was identified as the odh gene. Transformed Escherichia coli cells overproduced NAD+-dependent op...
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Main Authors: | , |
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Format: | Artigo |
Language: | Inglês |
Published: |
1995
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Subjects: | |
Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC167592/ https://ncbi.nlm.nih.gov/pubmed/7487048 |
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