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Codeine-binding RNA aptamers and rapid determination of their binding constants using a direct coupling surface plasmon resonance assay

RNA aptamers that bind the opium alkaloid codeine were generated using an iterative in vitro selection process. The binding properties of these aptamers, including equilibrium and kinetic rate constants, were determined through a rapid, high-throughput approach using surface plasmon resonance (SPR)...

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Bibliografski detalji
Glavni autori: Win, Maung Nyan, Klein, Joshua S., Smolke, Christina D.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2006
Teme:
RNA
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1636496/
https://ncbi.nlm.nih.gov/pubmed/17038331
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl718
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