Isoelectric focusing technology quantifies protein signaling in 25 cells

A previously undescribed isoelectric focusing technology allows cell signaling to be quantitatively assessed in <25 cells. High-resolution capillary isoelectric focusing allows isoforms and individual phosphorylation forms to be resolved, often to baseline, in a 400-nl capillary. Key to the metho...

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Hlavní autoři: O'Neill, Roger A., Bhamidipati, Arunashree, Bi, Xiahui, Deb-Basu, Debabrita, Cahill, Linda, Ferrante, Jason, Gentalen, Erik, Glazer, Marc, Gossett, John, Hacker, Kevin, Kirby, Celeste, Knittle, James, Loder, Robert, Mastroieni, Catherine, MacLaren, Michael, Mills, Thomas, Nguyen, Uyen, Parker, Nineveh, Rice, Audie, Roach, David, Suich, Daniel, Voehringer, David, Voss, Karl, Yang, Jade, Yang, Tom, Vander Horn, Peter B.
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 2006
Témata:
Biological Sciences
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1618307/
https://ncbi.nlm.nih.gov/pubmed/17053065
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0607973103
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