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Crowding and Confinement Effects on Protein Diffusion In Vivo

The first in vivo measurements of a protein diffusion coefficient versus cytoplasmic biopolymer volume fraction are presented. Fluorescence recovery after photobleaching yields the effective diffusion coefficient on a 1-μm-length scale of green fluorescent protein within the cytoplasm of Escherichia...

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Main Authors: Konopka, Michael C., Shkel, Irina A., Cayley, Scott, Record, M. Thomas, Weisshaar, James C.
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2006
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC1595386/
https://ncbi.nlm.nih.gov/pubmed/16923878
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.01982-05
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