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Sensitive, Seminested PCR Amplification of VP1 Sequences for Direct Identification of All Enterovirus Serotypes from Original Clinical Specimens

A reverse transcription-seminested PCR (RT-snPCR) assay was developed for the detection and identification of enterovirus (EV) RNA in clinical specimens. Three conserved protein motifs were identified by aligning the VP3 and VP1 sequences of prototype EV strains. Consensus degenerate primers were de...

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主要な著者: Nix, W. Allan, Oberste, M. Steven, Pallansch, Mark A.
フォーマット: Artigo
言語:Inglês
出版事項: American Society for Microbiology 2006
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC1594621/
https://ncbi.nlm.nih.gov/pubmed/16891480
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00542-06
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