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Proteasomal Proteomics: Identification of Nucleotide-sensitive Proteasome-interacting Proteins by Mass Spectrometric Analysis of Affinity-purified Proteasomes

Ubiquitin-dependent proteolysis is catalyzed by the 26S proteasome, a dynamic complex of 32 different proteins whose mode of assembly and mechanism of action are poorly understood, in part due to the difficulties encountered in purifying the intact complex. Here we describe a one-step affinity metho...

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Hlavní autoři: Verma, Rati, Chen, Stephen, Feldman, Renny, Schieltz, David, Yates, John, Dohmen, Juergen, Deshaies, Raymond J.
Médium: Artigo
Jazyk:Inglês
Vydáno: The American Society for Cell Biology 2000
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC15004/
https://ncbi.nlm.nih.gov/pubmed/11029046
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