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Sequencing RNA by a combination of exonuclease digestion and uridine specific chemical cleavage using MALDI-TOF.

The determination of DNA sequences by partial exonuclease digestion followed by Matrix-Assisted Laser Desorption Time of Flight Mass Spectrometry (MALDI-TOF) is a well established method. When the same procedure is applied to RNA, difficulties arise due to the small (1 Da) mass difference between th...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Tolson, D A, Nicholson, N H
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1998
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC147280/
https://ncbi.nlm.nih.gov/pubmed/9421498
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