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Quantitative profiling of differentiation-induced microsomal proteins using isotope-coded affinity tags and mass spectrometry

An approach to the systematic identification and quantification of the proteins contained in the microsomal fraction of cells is described. It consists of three steps: (1) preparation of microsomal fractions from cells or tissues representing different states; (2) covalent tagging of the proteins wi...

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Autori principali: Han, David K., Eng, Jimmy, Zhou, Huilin, Aebersold, Ruedi
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2001
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1444949/
https://ncbi.nlm.nih.gov/pubmed/11581660
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt1001-946
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