A real-time PCR-based method for determining the surface coverage of thiol-capped oligonucleotides bound onto gold nanoparticles

Here we report a real-time PCR-based method for determining the surface coverage of dithiol-capped oligonucleotides bound onto gold nanoparticles alone and in tandem with antibody. The detection of gold nanoparticle-bound DNA is accomplished by targeting the oligonucleotide with primer and probe bin...

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Bibliographic Details
Main Authors: Kim, Eun-Young, Stanton, Jennifer, Vega, Rafael A., Kunstman, Kevin J., Mirkin, Chad A., Wolinsky, Steven M.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2006
Subjects:
Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1440878/
https://ncbi.nlm.nih.gov/pubmed/16617142
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl147
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