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Calcium-binding parameter of Bacillus amyloliquefaciens alpha-amylase determined by inactivation kinetics.
The irreversible thermal inactivation and the thermodynamics of calcium ion binding of Bacillus amyloliquefaciens alpha-amylase in the absence of substrates were studied. The enzyme inactivation on heating was apparently followed by first-order kinetics. The enzyme was stabilized with an increased c...
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Main Authors: | , |
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Format: | Artigo |
Language: | Inglês |
Published: |
2002
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Subjects: | |
Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1222611/ https://ncbi.nlm.nih.gov/pubmed/12049626 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20011436 |
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