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Reconstitution of the quinoprotein methanol dehydrogenase from inactive Ca(2+)-free enzyme with Ca2+, Sr2+ or Ba2+.

The reconstitution of active holoenzyme containing calcium from inactive calcium-free methanol dehydrogenase, isolated from a moxA mutant of Methylobacterium extorquens, has a pH optimum of about pH 10, with a well defined pK for the process at pH 9.3. Two Ca2+ ions were irreversibly incorporated pe...

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Autors principals: Goodwin, M G, Avezoux, A, Dales, S L, Anthony, C
Format: Artigo
Idioma:Inglês
Publicat: 1996
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1217864/
https://ncbi.nlm.nih.gov/pubmed/8920988
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